name: paperconfig description: Create and validate paperconfig.yaml files for integrating omics publication data (RNA-seq, proteomics, metabolomics, microarray) into the knowledge graph. Use when adding a new paper's differential expression data. argument-hint: [paper-directory-name] user-invocable: true allowed-tools: Read, Grep, Glob, Write, Edit, Bash(uv ), Bash(python )

Paperconfig YAML Instructions

How to create a paperconfig.yaml file for integrating omics publication data into the knowledge graph.

Overview

Each publication with differential expression or omics data gets its own paperconfig.yaml file under data/Prochlorococcus/papers_and_supp/<AuthorName Year>/. This config tells the OMICSAdapter how to parse supplementary data tables and create knowledge graph nodes and edges.

Directory Setup

Create a folder: data/Prochlorococcus/papers_and_supp/<AuthorName Year>/
Place the publication PDF in this folder
Place the supplementary data CSV file(s) in this folder (or a subfolder)
Create paperconfig.yaml in the same folder

YAML Structure

A paperconfig has three top-level sections under publication::

publication:
  papername: "<Author Year>"
  papermainpdf: "data/Prochlorococcus/papers_and_supp/<AuthorName Year>/<filename>.pdf"

  environmental_conditions:
    # ... (optional, see below)

  supplementary_materials:
    # ... (required)

1. Publication Metadata (Required)

publication:
  papername: "Author Year"
  papermainpdf: "data/Prochlorococcus/papers_and_supp/<folder>/<pdf_filename>.pdf"

papername: Short citation label (e.g., "Biller 2016"). Used for display only.
papermainpdf: Relative path to the publication PDF. The adapter extracts metadata (title, DOI, authors, abstract) from this PDF automatically using an LLM-based extractor.

2. Environmental Conditions (Optional)

Define environmental conditions when the experiment varies an environmental factor (light, gas, salinity, nutrients, growth state) rather than a biological organism. Each condition becomes a node in the knowledge graph and can be used as the source of affects_expression_of edges (instead of an organism).

  environmental_conditions:
    <unique_condition_id>:
      condition_type: "<type>"    # e.g., gas_shock, salt_stress, light_stress, growth_medium, growth_state, coculture
      name: "<human-readable name>"
      description: "<detailed description of the condition>"
      # Include any relevant parameters:
      medium: "<growth medium>"
      temperature: "<temp>"
      light_condition: "<light type>"
      light_intensity: "<intensity>"
      # Gas-specific:
      co2_level: "<level>"
      oxygen_level: "<level>"
      # Salinity-specific:
      salinity: "<level>"

Rules:

The <unique_condition_id> is a local key used to link conditions to analyses (via environmental_treatment_condition_id and environmental_control_condition_id).
Use a naming convention: <authorlastname>_<year>_<short_description> (e.g., bagby_2015_low_co2).
All property keys are stored as-is on the environmental_condition node.
If the experiment compares organisms (e.g., coculture vs axenic), you may still define environmental conditions but they are not strictly required -- you can use treatment_organism/treatment_taxid on the analysis instead.

3. Supplementary Materials (Required)

Each supplementary table is a keyed entry containing a filename and one or more statistical analyses.

  supplementary_materials:
    <table_key>:
      type: csv
      filename: "data/Prochlorococcus/papers_and_supp/<folder>/<data_file>.csv"
      statistical_analyses:
        - <analysis_1>
        - <analysis_2>
        # ... multiple analyses can reference the same file

<table_key>: A descriptive key for the table (e.g., supp_table_1, supp_table_2_timepoint_A).
type: Always csv.
filename: Relative path to the data CSV file.
Multiple analyses can share the same file if different columns represent different comparisons (e.g., different timepoints or conditions in the same spreadsheet).

4. Statistical Analysis Fields

Each analysis entry describes one differential expression comparison.

Required Fields

| Field | Description | Examples | |-------|-------------|----------| | type | Omics data type | RNASEQ, MICROARRAY, PROTEOMICS, METABOLOMICS | | name | Human-readable description of the analysis | "DE Analysis of Prochlorococcus MED4 coculture vs axenic at 20h" | | id | Unique identifier for this analysis | DE_coculture_vs_axenic_med4_20h | | test_type | Statistical method used | DESeq2, edgeR, Rockhopper, Affymetrix microarray, DESeq | | control_condition | Baseline/reference condition | "Axenic", "Time 0 (pre-shock)", "Normal seawater (3.8% salt)" | | treatment_condition | Experimental/test condition | "Coculture with Alteromonas HOT1A3", "Salt-acclimated (5% salt)" | | organism | Organism being profiled | "Prochlorococcus MED4", "Alteromonas macleodii MIT1002" | | name_col | Column name in CSV containing the gene/protein identifier | "Gene", "Locus tag2", "Gene ID", "Synonym", "ID" | | logfc_col | Column name in CSV containing the log2 fold change value | "log2FoldChange", "logFC", "0.036%_CO2_21%_O2 FC" |

Optional Fields

| Field | Description | When to Use | |-------|-------------|-------------| | adjusted_p_value_col | Column name for adjusted p-value | When the CSV has a p-value column (e.g., "padj", "FDR", "q Value") | | experimental_context | Other factors held constant | Always helpful; describes medium, light, temperature, etc. | | timepoint | Time point of measurement | For time-course experiments (e.g., "20h", "48h", "-12h", "24h vs 12h") | | skip_rows | Number of header rows to skip when reading CSV | When the CSV has extra header rows before the data columns (e.g., 3) | | pvalue_asterisk_in_logfc | Boolean, true if significance is marked by * appended to fold-change values | When there is no separate p-value column and the fold-change column has asterisks (e.g., "2.5*") | | prefiltered | Boolean, true if the table only contains significant results | When authors pre-filtered the table to significant genes only | | pvalue_threshold | Adjusted p-value threshold used/stated in the publication (e.g., 0.05) | When the paper states a specific significance cutoff | | logfc_threshold | Absolute log2 fold-change threshold used/stated in the publication (e.g., 0.8, 1.0) | When the paper states a specific fold-change cutoff |

Note on tables where all values are significant: Some supplementary tables only list genes that are already significantly differentially expressed (pre-filtered by the authors). In these cases, set prefiltered: true on the analysis. This tells the adapter that all rows are significant. You may also omit adjusted_p_value_col and pvalue_asterisk_in_logfc if not applicable.

Note on significance thresholds: If the paper or table legend states specific significance criteria (e.g., "adjusted p-value < 0.05 and |log2FC| >= 1"), record these as pvalue_threshold and logfc_threshold. The adapter uses these to mark edges as significant or not. If the paper does not state thresholds, omit these fields — the adapter can apply default thresholds from its constructor settings.

Edge Source Fields (One Set Required)

The adapter needs to determine what causes the expression change. You must provide one of these two sets:

Option A -- Organism as cause (e.g., coculture experiments):

| Field | Description | Example | |-------|-------------|---------| | treatment_organism | Name of the organism causing the effect | "Alteromonas macleodii HOT1A3" | | treatment_taxid | NCBI Taxonomy ID of the treatment organism | 28108 |

Option B -- Environmental condition as cause (e.g., stress experiments):

| Field | Description | Example | |-------|-------------|---------| | environmental_treatment_condition_id | References a key from environmental_conditions | bagby_2015_low_co2 | | environmental_control_condition_id | (Optional) References the control condition key | alhosani_2015_seawater_control |

If environmental_treatment_condition_id is present, the adapter uses the environmental condition node as the edge source. Otherwise it falls back to the organism node from treatment_taxid.

Choosing Between Organism and Environmental Condition Edges

Use organism edges (treatment_organism + treatment_taxid) when:

The experiment adds or removes a biological organism (e.g., coculture with a heterotroph, viral infection)
The "cause" of expression change is the presence of another organism

Use environmental condition edges (environmental_treatment_condition_id) when:

The experiment varies a physical or chemical factor (light, gas, salinity, nutrients, temperature)
The experiment compares growth states or phases (planktonic vs biofilm, exponential vs stationary)
You want the condition details (CO2 level, salinity, etc.) stored as a node with rich properties

CSV Data File Requirements

Must be a valid CSV file readable by pandas
Must contain at minimum:
- A column with gene/protein identifiers (referenced by name_col)
- A column with log2 fold-change values (referenced by logfc_col)
Optionally: a column with adjusted p-values (referenced by adjusted_p_value_col)
Gene identifiers should ideally be locus tags or gene IDs that can be matched to existing gene nodes in the knowledge graph
Rows with empty/NA gene identifiers or non-numeric fold-change values are automatically skipped

Complete Examples

Example 1: Coculture Experiment (Organism as Cause)

publication:
  papername: "Aharonovich 2016"
  papermainpdf: "data/Prochlorococcus/papers_and_supp/Aharonovich 2016/paper.pdf"

  supplementary_materials:
    supp_table_1:
      type: csv
      filename: "data/Prochlorococcus/papers_and_supp/Aharonovich 2016/de_genes_med4_t20h.csv"
      statistical_analyses:
        - type: RNASEQ
          name: "DE of Prochlorococcus MED4 in Coculture with Alteromonas HOT1A3 vs Axenic at 20h"
          id: DE_coculture_vs_axenic_med4_20h
          test_type: Rockhopper
          control_condition: Axenic
          treatment_condition: "Coculture with Alteromonas HOT1A3"
          experimental_context: "in Pro99 medium under continuous light"
          timepoint: 20h
          organism: "Prochlorococcus MED4"
          treatment_taxid: 28108
          treatment_organism: "Alteromonas macleodii HOT1A3"
          name_col: Synonym
          logfc_col: "log 2 fold change coculture/axenic"
          adjusted_p_value_col: "q Value"

Example 2: Environmental Stress (Condition as Cause)

publication:
  papername: "Al-Hosani 2015"
  papermainpdf: "data/Prochlorococcus/papers_and_supp/Al-Hosani 2015/paper.pdf"

  environmental_conditions:
    alhosani_2015_seawater_control:
      condition_type: growth_medium
      name: "Normal seawater control (3.8% salt)"
      medium: "PRO99 natural seawater medium"
      temperature: 22C
      light_condition: continuous_light
      light_intensity: "46 umol photons m-2 s-1"
      salinity: "3.8% w/v NaCl"
      description: "Axenic Prochlorococcus AS9601 in PRO99 medium, normal salinity"
    alhosani_2015_salt_acclimation:
      condition_type: salt_stress
      name: "Salt-acclimated (5% salt)"
      medium: "PRO99 natural seawater medium with added NaCl"
      temperature: 22C
      light_condition: continuous_light
      light_intensity: "46 umol photons m-2 s-1"
      salinity: "5% w/v NaCl"
      description: "Axenic Prochlorococcus AS9601 acclimated to 5% NaCl"

  supplementary_materials:
    supp_table_3:
      type: csv
      filename: "data/Prochlorococcus/papers_and_supp/Al-Hosani 2015/de_genes_salt.csv"
      statistical_analyses:
        - type: RNASEQ
          name: "DE of Prochlorococcus AS9601 under salt acclimation vs normal seawater"
          id: DE_salt_acclimated_vs_seawater_AS9601
          test_type: DESeq
          control_condition: "Normal seawater (3.8% salt)"
          treatment_condition: "Salt-acclimated (5% salt)"
          environmental_control_condition_id: alhosani_2015_seawater_control
          environmental_treatment_condition_id: alhosani_2015_salt_acclimation
          experimental_context: "Axenic cells in PRO99 medium at 22C under continuous light"
          organism: "Prochlorococcus AS9601"
          name_col: "Gene id"
          logfc_col: log2FoldChange
          adjusted_p_value_col: padj

Example 3: Multiple Analyses from One File (Time Course)

When a single CSV contains columns for multiple timepoints:

  supplementary_materials:
    supp_table_2:
      type: csv
      filename: "data/.../PRO_DE_genes.csv"
      statistical_analyses:
        - type: RNASEQ
          name: "DE Prochlorococcus NATL2A coculture vs axenic (2h)"
          id: DE_coculture_vs_axenic_NATL2A_2h
          test_type: DESeq2
          control_condition: Axenic
          treatment_condition: "Co-culture with Alteromonas macleodii MIT1002"
          timepoint: 2h
          organism: "Prochlorococcus NATL2A"
          treatment_taxid: 28108
          treatment_organism: "Alteromonas macleodii MIT1002"
          name_col: Original_NCBI_ID
          logfc_col: "2 hours"
          skip_rows: 3
          pvalue_asterisk_in_logfc: true
        - type: RNASEQ
          name: "DE Prochlorococcus NATL2A coculture vs axenic (12h)"
          id: DE_coculture_vs_axenic_NATL2A_12h
          test_type: DESeq2
          control_condition: Axenic
          treatment_condition: "Co-culture with Alteromonas macleodii MIT1002"
          timepoint: 12h
          organism: "Prochlorococcus NATL2A"
          treatment_taxid: 28108
          treatment_organism: "Alteromonas macleodii MIT1002"
          name_col: Original_NCBI_ID
          logfc_col: "12 hours"
          skip_rows: 3
          pvalue_asterisk_in_logfc: true

Example 4: Multiple Tables from Same Paper

When a paper has separate CSV files for different organisms or experiments:

  supplementary_materials:
    supp_table_pro:
      type: csv
      filename: "data/.../pro_de_genes.csv"
      statistical_analyses:
        - type: RNASEQ
          # ... Prochlorococcus analysis
    supp_table_alt:
      type: csv
      filename: "data/.../alt_de_genes.csv"
      statistical_analyses:
        - type: RNASEQ
          # ... Alteromonas analysis

Example 5: Environmental Stress with Multiple Biological Contexts in One File

When a single CSV has columns for different biological contexts (e.g., axenic and coculture) at multiple timepoints, all comparing the same environmental treatment vs control:

publication:
  papername: "Biller 2018"
  papermainpdf: "data/Prochlorococcus/papers_and_supp/Biller 2018/paper.pdf"

  environmental_conditions:
    biller_2018_diel_ld_control:
      condition_type: growth_medium
      name: "13:11 diel light:dark cycle control"
      medium: Pro99 natural seawater medium
      temperature: 24C
      light_condition: "13:11 light:dark cycle with simulated dawn and dusk"
      light_intensity: "55 umol photons m-2 s-1"
      description: "Prochlorococcus NATL2A maintained under a 13:11 light:dark diel cycle"
    biller_2018_extended_darkness:
      condition_type: light_stress
      name: "Extended darkness"
      medium: Pro99 natural seawater medium
      temperature: 24C
      light_condition: continuous_darkness
      light_intensity: "0 umol photons m-2 s-1"
      description: "Cultures shifted to continuous darkness at expected sunrise"

  supplementary_materials:
    supp_table_s3:
      type: csv
      filename: "data/.../de_genes.csv"
      statistical_analyses:
        # Axenic condition at 1h
        - type: RNASEQ
          name: "DE Prochlorococcus NATL2A axenic in extended darkness vs diel (1h)"
          id: DE_extended_darkness_vs_diel_axenic_NATL2A_1h
          test_type: DESeq2
          control_condition: "13:11 diel light:dark cycle"
          treatment_condition: "Extended darkness"
          environmental_control_condition_id: biller_2018_diel_ld_control
          environmental_treatment_condition_id: biller_2018_extended_darkness
          experimental_context: "Axenic Prochlorococcus NATL2A in Pro99 at 24C"
          timepoint: "1h extended darkness"
          organism: Prochlorococcus NATL2A
          name_col: "NCBI ID"
          logfc_col: "Axenic, 36 hours"
          pvalue_asterisk_in_logfc: true
        # Coculture condition at 1h (same file, different logfc_col)
        - type: RNASEQ
          name: "DE Prochlorococcus NATL2A coculture in extended darkness vs diel (1h)"
          id: DE_extended_darkness_vs_diel_coculture_NATL2A_1h
          test_type: DESeq2
          control_condition: "13:11 diel light:dark cycle"
          treatment_condition: "Extended darkness"
          environmental_control_condition_id: biller_2018_diel_ld_control
          environmental_treatment_condition_id: biller_2018_extended_darkness
          experimental_context: "Prochlorococcus NATL2A co-cultured with Alteromonas macleodii MIT1002 in Pro99 at 24C"
          timepoint: "1h extended darkness"
          organism: Prochlorococcus NATL2A
          name_col: "NCBI ID"
          logfc_col: "Co-culture, 36 hours"
          pvalue_asterisk_in_logfc: true
        # ... repeat for additional timepoints

Registration

After creating the paperconfig, register it in the pipeline:

Add the path to data/Prochlorococcus/papers_and_supp/paperconfig_files.txt (one path per line)
Or instantiate it directly in create_knowledge_graph.py:

omics = OMICSAdapter(config_file="data/Prochlorococcus/papers_and_supp/<AuthorName Year>/paperconfig.yaml")
omics.download_data(cache=True)
bc.write_nodes(omics.get_nodes())
bc.write_edges(omics.get_edges())

Validation Checklist

Before submitting a paperconfig:

[ ] papermainpdf path points to an existing PDF file
[ ] All filename paths point to existing CSV files
[ ] name_col matches an actual column name in the CSV
[ ] logfc_col matches an actual column name in the CSV
[ ] adjusted_p_value_col (if specified) matches an actual column name in the CSV
[ ] Each analysis has either (treatment_organism + treatment_taxid) or environmental_treatment_condition_id
[ ] If using environmental_treatment_condition_id, the referenced key exists under environmental_conditions
[ ] Each analysis id is unique within the file
[ ] skip_rows is set correctly if the CSV has extra header rows
[ ] Gene/protein identifiers in name_col use locus tags or IDs that can match existing gene nodes
[ ] prefiltered is set to true if the table only contains significant results
[ ] pvalue_threshold and logfc_threshold are set if the paper states specific significance criteria

Workflow

When the user invokes this skill with a paper directory name (e.g., /paperconfig "Author Year"):

Look for the paper directory under data/Prochlorococcus/papers_and_supp/$ARGUMENTS/
List all files in that directory (PDF, CSV, XLSX, TXT, DOCX, etc.)
Read any legend/description text files first -- these explain what the supplementary tables contain (column meanings, significance criteria, experimental details)
Read the CSV file headers and a few sample rows to identify available columns and data format (check for asterisks in fold-change values, presence of p-value columns, skip rows, etc.)
Read the PDF to understand the experiment (organisms, conditions, methods, statistical approach)
Draft the paperconfig.yaml following the schema above
Run the validation script (see below) to check all paths, columns, references, and ID uniqueness
Register the config in paperconfig_files.txt

Validation Script

After creating the paperconfig, always run the validation script:

uv run python .claude/skills/paperconfig/validate_paperconfig.py "data/Prochlorococcus/papers_and_supp/<Author Year>/paperconfig.yaml"

This script checks:

YAML is parseable and has required top-level structure
PDF file exists
All CSV files exist and are readable
Column names (name_col, logfc_col, adjusted_p_value_col) match actual CSV headers
skip_rows is applied correctly when reading CSV headers
Each analysis has all required fields (type, name, id, test_type, control_condition, treatment_condition, organism, name_col, logfc_col)
Each analysis has either (treatment_organism + treatment_taxid) or environmental_treatment_condition_id
Environmental condition ID references resolve to keys in environmental_conditions
All analysis IDs are unique
logfc_col values look numeric (warns on non-numeric values)
type is one of: RNASEQ, MICROARRAY, PROTEOMICS, METABOLOMICS

The script exits with code 0 on success, 1 on validation failure.

Paperconfig YAML Configuration for Omics Data